Or your ability to do science, or to graduate, or any other things. The only thing a failed experiment can tell you is that the experiment did not work. An experiment not working is VERY different from an experiment not giving you the data you want. An experiment not working is an SDS-Page Gel not running for the 4th week in a row, all for different reasons. The experiment not workings is not getting a signal on your membrane and you don’t know if its because the protein didn’t transfer, the antibody concentration is correct or the 2ndary is off.
Thats not entirely true. I know the protein transfer efficiency was enough to have protein1 detected. I know that 1 of the secondaries work fine. That is all I know. Oh that have 3 days of washing and probing, ponceaus S (1% w/v) picks up ZERO bands on the membrane.
So for all the newbie graduate students that started over the summer and the last couple weeks. The title was kinda for you. But mostly its the mantra I need to tell myself on an hourly basis as I struggle with biochemistry.
I will publish this damn story and I will graduate.